Mol Cancer Res. 2007 Sep;5(9):943-55.
Ursolic acid inhibits STAT3 activation pathway leading to suppression of proliferation and chemosensitization of human multiple myeloma cells.
Source
Cytokine Research Laboratory, Department of Experimental Therapeutics, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Box 143, Houston, TX 77030, USA.
FIGURE 4.
A. Ursolic acid enhances the expression of proapoptotic Bax and Bak proteins. U266 cells (2 × 106/mL) were treated with 50 μmol/L ursolic acid for the indicated time intervals, after which whole-cell extracts were prepared and 30-μg portions of those extracts were resolved on 10% SDS-PAGE and probed against Bax and Bak antibodies. The same blots were stripped and reprobed with β-actin antibody to verify equal protein loading. B. Ursolic acid suppresses STAT3 regulated antiapoptotic gene products. U266 cells (2 × 106/mL) were treated with 50 μmol/L ursolic acid for the indicated time intervals, after which whole-cell extracts were prepared and 30-μg portions of those extracts were resolved on 10% SDS-PAGE, membrane sliced according to molecular weight, and probed against cyclin D1, Bcl-2, Bcl-XL, survivin, and VEGF antibodies. The same blots were stripped and reprobed with β-actin antibody to verify equal protein loading. C.Ursolic acid suppresses the expression of Mcl-1 protein. U266 cells (2 × 106/mL) were treated with 50 μmol/L ursolic acid for the indicated time intervals, after which whole-cell extracts were prepared and 30-μg portions of those extracts were resolved on 10% SDS-PAGE and probed against Mcl-1 antibodies. The same blots were stripped and reprobed with β-actin antibody to verify equal protein loading. D. Ursolic acid inhibits gene expression. U266 cells (4 × 106/mL) were treated with ursolic acid (50 μmol/L) for the indicated time points, and total RNA was extracted and examined for expression ofcyclin D1, Bcl-2, and Bcl-xL by RT-PCR. Glyceraldehyde-3-phosphate dehydrogenase was used as an internal control to show equal RNA loading.
Figure 6A - Control, UA, and Bortezomib images are the same as Control, Capsaicin, Velcade
images in Figure 6A and 6B of PMID 17505005
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